A reverse transcriptase-PCR based assay for in-vitro antibiotic susceptibility testing of Chlamydia pneumoniae
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چکیده
منابع مشابه
A reverse transcriptase-PCR based assay for in-vitro antibiotic susceptibility testing of Chlamydia pneumoniae.
Infections caused by Chlamydia spp are an important cause of human disease, and the accuracy and reproducibility of antimicrobial susceptibility tests for these bacteria could have considerable clinical implications. We have developed a reverse transcriptase PCR (RT-PCR) based method to determine the antibiotic susceptibility of Chlamydia spp., and compared this with conventional tests using im...
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ES cells. This phenomenon has been observed in our laboratory in a number of cases. Clones putatively identified as homologous recombinants by Southern analysis can be quickly checked for random insertion of extra copies of the targeting vector by PCR. Given the small difference in size between the two alleles and use of only two primers common to both WT and mutant DNA, the amplification rate ...
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Chlamydia species are susceptible in vitro to certain antibiotics, and various tetracyclines, macrolides, and fluoroquinolones are used for the treatment of Chlamydia trachomatis infections. Less is known about the in vitro susceptibility and chemical response to infection with Chlamydia pneumoniae. Results of in vitro studies from the literature and from my laboratory employing a tissue cultur...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 1996
ISSN: 0305-7453,1460-2091
DOI: 10.1093/jac/37.4.677